Journal: Journal of Biological Engineering

Article Title: Advanced biomanufacturing and evaluation of adeno-associated virus

doi: 10.1186/s13036-024-00409-4

Figure Lengend Snippet: Development and optimization of anion exchange purification using liquid chromatography (LC). The 80–140 mL of cell lysis from 20–100 mL of VPC pellet was loaded to the 1-mL or 5-mL columns. The representative LC profile of AAV-DJ8 was described here but four serotypes of AAV2, 5, DJ and DJ8 were tested using the developed purification strategy. Equilibration buffer: 25 mM Tris–HCl, pH 9.0. Elution Buffer A: 25 mM Tris–HCl, 20 mM NaCl, pH 9.0. Elution buffer B: 25 mM Tris–HCl, 1 M NaCl, pH 9.0. Flow rate: 1.0 mL/min. A Stepwise elution of AAV-DJ8, 80-mL AAV lysis from 20-mL VPC pellet, in 1-mL EconoFit Nuvia aPrime 4A column. B Stepwise elution of AAV-DJ8, 140-mL AAV lysis from 100-mL VPC pellet, in 1-mL EconoFit Nuvia aPrime 4A column. C Stepwise elution of AAV-DJ8, 100-mL AAV lysis from 50-mL VPC pellet, using 1-mL Foresight Nuvia HPQ column, which can be scaled up from 1-mL column to 5-mL and 25-mL columns. D Stepwise elution of AAV-DJ8, 100-mL AAV lysis from 50-mL VPC pellet, using 5-mL Foresight Nuvia HPQ column

Article Snippet: Multiple commercial columns for AAV purification have been evaluated in this study (Table ), including Cytiva HiTrap Q Sepharose XL strong anion exchange column, Cytiva Sepharose Fast Flow anion exchange column, Cytiva HiTrap AVB Sepharose column, Bio-Rad Foresight Nuvia HPQ column, and Bio-Rad EconoFit Nuria aPrime 4A.

Techniques: Purification, Liquid Chromatography, Lysis

Journal: Pediatrics

Article Title: The Safe Environment for Every Kid Model: Impact on Pediatric Primary Care Professionals

doi: 10.1542/peds.2010-1845

Figure Lengend Snippet: Thematic HPQ Scale Difference Scores From Baseline Comparing SEEK and Control at 6, 18, and 36 Months

Article Snippet: In the SEEK group, HPQ scores increased from baseline to follow-up for every domain and at every time point.

Techniques: Control

Journal: Pediatrics

Article Title: The Safe Environment for Every Kid Model: Impact on Pediatric Primary Care Professionals

doi: 10.1542/peds.2010-1845

Figure Lengend Snippet: Topic HPQ Scale Difference Scores From Baseline Comparing SEEK and Control at 6, 18, and 36 Months

Article Snippet: In the SEEK group, HPQ scores increased from baseline to follow-up for every domain and at every time point.

Techniques: Control